| African Cemetery No. 2 | Lexington | Site | 2004 |
| Allen, James House | Lexington | Building | 1982 |
| Ashland NHL | Lexington | Building | 1966 |
| Ashland Park Historic District | Lexington | District | 1986 |
| Athens Historic District | Athens | District | 1979 |
| Barton, Abraham, House | Lexington | Building | 1977 |
| Basye, T. D., House | Lexington | Building | 1987 |
| Bates Log House | Lexington | Building | 1982 |
| Beck, James Burnie, House | Lexington | Building | 1982 |
| Bell Court Neighborhood Historic District | Lexington | District | 1980 |
| Bell Place | Lexington | Building | 1978 |
| Bell, John, House | Lexington | Building | 1983 |
| Boone Creek Rural Historic District | Lexington | District | 1994 |
| Botherum | Lexington | Building | 1973 |
| Bowman Houses | Lexington | Building | 1979 |
| Bowman Mill Road Rural Historic District | Lexington | District | 1999 |
| Brand-Barrow House | Lexington | Building | 1976 |
| Breckinridge, John C., Memorial | Lexington | Object | 1997 |
| Brown, Joshua, House | Lexington | Building | 1979 |
| Buenna Hill | Centerville | Building | 1983 |
| Burrier, John, House | Lexington | Building | 1983 |
| Cadentown School | Lexington | Building | 2006 |
| Cave Place | Lexington | Building | 1980 |
| Cave Spring | Lexington | Building | 1978 |
| Central Christian Church | Lexington | Building | 1979 |
| Central Kentucky Blue Grass Seed Co. | Lexington | Building | 2005 |
| Chandler Normal School Building and Webster Hall | Lexington | Building | 1980 |
| Christ Church Episcopal | Lexington | Building | 1976 |
| Clark Hardware Company Building | Lexington | Building | 1979 |
| Clark, John, House | Lexington | Building | 1980 |
| Clay, Henry, Law Office | Lexington | Building | 1971 |
| Cleveland-Rogers House | Lexington | Building | 1980 |
| Cloud House | Lexington | Building | 1982 |
| Conant, William, House | Lexington | Building | 1991 |
| Confederate Soldier Monument in Lexington | Lexington | Object | 1997 |
| Constitution Historic District | Lexington | District | 1982 |
| Corinthia | Centerville | Building | 1983 |
| DeLong Agricultural Implements Warehouse | Lexington | Building | 1980 |
| Delta | Lexington | Building | 1978 |
| Douglass School | Lexington | Building | 1998 |
| Downtown Commercial District | Lexington | District | 1983 |
| Edgewood | Lexington | Building | 2016 |
| Elam Mound Archeological Site | Lexington | Site | 1975 |
| Elley Villa | Lexington | Building | 1978 |
| Elmendorf | Lexington | District | 2023 |
| Elsmere Park Historic District | Lexington | District | 1976 |
| Episcopal Burying Ground and Chapel | Lexington | Site | 1976 |
| Fairlawn | Lexington | Building | 1976 |
| Fayette National Bank (Boundary Increase) | Lexington | Building | 2014 |
| Fayette National Bank Building | Lexington | Building | 1980 |
| Fayette Safety Vault and Trust Company Building | Lexington | Building | 1980 |
| Featherston, Edmonson and Clark Houses | Lexington | Building | 1983 |
| First African Baptist Church | Lexington | Building | 1986 |
| First Presbyterian Church | Lexington | Building | 1974 |
| Floral Hall | Lexington | Building | 1977 |
| Grant, George W., House | Lexington | Building | 1980 |
| Gratz Park Historic District | Lexington | District | 1973 |
| Graves Tavern | Versailles | Building | 1983 |
| Grimes House and Mill Complex | Lexington | Building | 1982 |
| Guilfoil Village Site (15FA176) | Athens | Site | 1985 |
| Hartland | Lexington | Building | 1987 |
| Hayes, Samuel T., House | Lexington | Building | 1980 |
| Headley, George, House | Lexington | Building | 1991 |
| Headley, Hal Price, Sr., House | Lexington | Building | 2010 |
| Helm Place | Lexington | Building | 1978 |
| Higgins Block | Lexington | Building | 1977 |
| Highland Hall | Lexington | Building | 1977 |
| Hollywood Terrace Historic District | Lexington | District | 2008 |
| Hurricane Hall | Lexington | Building | 1976 |
| Innes House | Centerville | Building | 1983 |
| January, Thomas, House | Lexington | Building | 1974 |
| Keeneland-Keeneland Racetrack NHL | Lexington | District | 1986 |
| Kennedy, Matthew, House | Lexington | Building | 1973 |
| Kinkead House | Lexington | Building | 1982 |
| Kinkead, Henry P., House | Lexington | Building | 1984 |
| Ladies' Confederate Memorial, The | Lexington | Object | 1997 |
| Lemon Hill | Lexington | Building | 1978 |
| Lemon, James, Houses | Lexington | Building | 1974 |
| Lewis Manor | Lexington | Building | 1976 |
| Lexington Cemetery and Henry Clay Monument | Lexington | Site | 1976 |
| Lexington City National Bank Building | Lexington | Building | 1980 |
| Lexington Dry Goods Company Building | Lexington | Building | 1988 |
| Lexington Herald Building | Lexington | Building | 1982 |
| Lexington Hospitality Motor Inn | Lexington | Building | 2024 |
| Lexington National Cemetery | Lexington | Site | 1998 |
| Lexington Veterans Administration Hospital | Lexington | District | 2012 |
| Lexington-Fayette County Government Building Block | Lexington | Building | 1983 |
| Liggett and Meyers Harpring Tobacco Storage Warehouse | Lexington | Building | 2013 |
| Liggett and Myers Tobacco Re-handling Facility | Lexington | Building | 2003 |
| Lincoln, Mary Todd, House | Lexington | Building | 1971 |
| Loudoun House | Lexington | Building | 1973 |
| Luigart Malt House | Lexington | Building | 2025 |
| Mansfield | Lexington | Building | 1982 |
| Mathews, Courtney, House | Lexington | Building | 2011 |
| Maxwell Place | Lexington | Building | 1982 |
| McAdams and Morford Building | Lexington | Building | 1973 |
| McCann, Benjamin, House | Lexington | Building | 1982 |
| McCann, Neal, House | Lexington | Building | 1982 |
| McCauley, John, House | Lexington | Building | 1980 |
| McConnell Springs | Lexington | Site | 1976 |
| McConnell, James, House | Lexington | Building | 1983 |
| McConnell, William, House | Lexington | Building | 1983 |
| McCracken-Wilgus House | Lexington | Building | 1978 |
| McGarvey, Dr. John, House | Lexington | Building | 1974 |
| McPheeters, Charles, House | Lexington | Building | 1974 |
| Mentelle House | Lexington | Building | 2025 |
| Mentelle Park | Lexington | District | 1985 |
| Middle Reaches of Boone Creek Rural Historic District | Lexington | District | 1996 |
| Miller Brothers Building | Lexington | Building | 1980 |
| Monsieur Giron's Confectionary | Lexington | Building | 1974 |
| Moore-Redd-Frazer House | Lexington | Building | 1976 |
| Morgan, John Hunt, Memorial | Lexington | Object | 1997 |
| Morton, Will, Tavern Stand | Lexington | Building | 1978 |
| Morton, William, House | Lexington | Building | 1975 |
| Mt. Horeb Archeological District | Lexington | District | 1998 |
| Mt. Horeb Earthworks, Unit A | Lexington | Site | 1975 |
| North Broadway-Short Street Historic District | Lexington | District | 1983 |
| North Limestone Commercial District | Lexington | District | 1983 |
| Northeast Residential Historic District | Lexington | District | 1985 |
| Northside Historic Residential District | Lexington | Building | 1979 |
| Northside Residential Historic District (Boundary Increase) | Lexington | District | 1982 |
| Odd Fellows Temple | Lexington | Building | 1980 |
| Old Morrison, Transylvania College NHL | Lexington | Building | 1966 |
| Opera House and Yates Bookshop Building | Lexington | Building | 1975 |
| Payne, Henry, House | Lexington | Building | 1983 |
| Pensacola Park Historic District | Lexington | District | 2020 |
| Peoples Federal Savings and Loan Association | Lexington | Building | 2016 |
| Pepper, James E., Distillery | Lexington | Building | 2009 |
| Pettit's, James, Mill | Ford | Building | 1983 |
| Poindexter, William, House | Lexington | Building | 1974 |
| Pope Villa | Lexington | Building | 2018 |
| Poplar Grove | Lexington | Building | 1982 |
| Price, Pugh, House | Lexington | Building | 1979 |
| Price, Williamson, House | Lexington | Building | 1979 |
| Ramsey, Lewis, Jr., House | Lexington | Building | 1991 |
| Randall Building Bogaert's Jewelry Store | Lexington | Building | 1982 |
| Redd Road Rural Historic District | Lexington | District | 1991 |
| Ridgely House | Lexington | Building | 1973 |
| Rockefeller Mound | Lexington | Site | 1998 |
| Rogers, Joseph Hale, House | Lexington | Building | 1979 |
| Rose Hill | Lexington | Building | 1974 |
| Russel, Robert, House | Centerville | Building | 1983 |
| Russell School | Lexington | Building | 2006 |
| Sayre Female Institute | Lexington | Building | 1982 |
| Schwert, Drs. George W. & Margaret, House | Lexington | Building | 2024 |
| Scott and Wilson Houses District | Lexington | District | 1974 |
| Second Presbyterian Church | Lexington | Building | 1980 |
| Shady Side | Lexington | Building | 1976 |
| Shelby Family Houses | Lexington | District | 1978 |
| Shryack, Frederick, House | Clintonville | Building | 1983 |
| Smith, Mitchell Baker, Company Building | Lexington | Building | 1980 |
| South Hill Historic District | Lexington | District | 1978 |
| Southeast Greyhound Line Building | Lexington | Building | 2014 |
| Southeast Lexington Residential and Commercial District | Lexington | District | 1984 |
| Southern Railway Passenger Depot | Lexington | Building | 1987 |
| Spindletop Farm | Lexington | District | 2012 |
| Spring Hill Farm | Lexington | Building | 1978 |
| Springview Farm | Lexington | District | 2012 |
| Steele, Drewsilla, House | Lexington | Building | 1991 |
| Stony Point | Lexington | Building | 1979 |
| Todd, William Lytle, House | Lexington | Building | 1979 |
| Trail's End Camp | Lexington | Site | 2019 |
| Trotter's Warehouse | Lexington | Building | 1976 |
| United States Post Office and Court House | Lexington | Building | 1999 |
| Victorian Commercial Block | Lexington | District | 1978 |
| Walnut Hill Presbyterian Church | Lexington | Building | 1973 |
| Warfield, Dr. Walter, Building | Lexington | Building | 1980 |
| Watkins, Thomas B., House | Lexington | Building | 1982 |
| Watt, Henry, House | Lexington | Building | 1977 |
| Waveland | Lexington | Building | 1971 |
| West Fayette County Rural Historic District | Lexington | District | 1991 |
| Western Suburb Historic District | Lexington | District | 1976 |
| Wolf Wile Department Store Building | Lexington | Building | 1996 |
| Woodland | Lexington | Building | 1975 |
| Woodlands Historic District | Lexington | District | 1983 |
| Woodstock | Lexington | Building | 1975 |
| Woodward Heights Neighborhood Historic District | Lexington | District | 1980 |
| Woolworth, F.W., Building | Lexington | Building | 2002 |
| Worley, Allen, and Foushee Houses | Lexington | Building | 1976 |
| Young, Charles, Park and Community Center | Lexington | Building | 2016 |